@article{QIMS154798,
author = {Wenyue Qin and Jiazhi Cao and Hong Wang and Wenwu Ling},
title = {Quantitative evaluation of key factors influencing hepatic oxygen saturation measurement by photoacoustic imaging in mice},
journal = {Quantitative Imaging in Medicine and Surgery},
volume = {16},
number = {7},
year = {2026},
keywords = {},
abstract = {Background: Photoacoustic imaging (PAI) has emerged as a promising non-invasive technique for assessing hepatic oxygen saturation (sO2). However, systematic evaluation of its baseline characteristics and influencing factors in normal liver tissue remains limited. This study aimed to establish a standardized PAI protocol for hepatic sO2 measurement in normal C57BL/6 mice and identify key factors contributing to measurement variability.Methods: A total of 48 healthy C57BL/6 mice (24 males and 24 females, aged 7–8 weeks) were used. Inter- and intra-observer reliability were assessed. The effects of multiple factors, including feeding status, hepatic lobe location, scanning plane, measurement approach (percutaneous vs. laparotomy), sex, region of interest (ROI) depth, and ROI size, on hepatic sO2 were quantitatively analyzed using a Vevo LAZR-X PAI system.Results: Excellent inter- and intra-observer reliability were observed. Hepatic sO2 exhibited significant variation across feeding states and hepatic lobe locations, between sexes, and between percutaneous and laparotomy measurements (all P},
issn = {2223-4306}, url = {https://qims.amegroups.org/article/view/154798}
}